Therefore, it’s worth further defining the effects of PBC on osteoporosis. However, to our knowledge, the role of PBC in ovariectomy (OVX)-induced osteoporosis and osteoblast-mediated bone formation remains unclear. Owing to reactive oxygen species (ROS) playing a pivotal role in the occurrence of different types of osteoporosis, PBC may prevent bone loss in OVX rats by reversing oxidative stress-induced damage to bone formation for strong anti-oxidative stress ability. ![]() Interestingly, some studies demonstrated that PBC is also protective in experimental models of neurotoxicity/neuropathology (Colle et al. Studies have reported that PBC could have a crucial role in preventing ethanol-induced liver fibrosis, treatment of erectile dysfunction and renal disease through inhibiting lipid peroxidation and oxidatively modifying low-density lipoprotein particles in vitro, circumnavigates endothelial impairment (Su et al. Probucol (PBC) is a cholesterol-lowering agent, but it has been receiving increasing attention for multiple reasons, including immunomodulation, anti-oxidation and neuroprotection (Iqbal et al. In addition, antioxidant drugs such as melatonin, alpha lipoic acid and resveratrol have beneficial effects on the differentiation and mineralization functions in osteoblast, as well as treatment of osteoporosis (Lu et al. Moreover, higher oxidative stress not only induces dramatic apoptosis in primary osteoblasts, but more importantly inhibits osteogenic differentiation (Baek et al. The level of oxidative stress will increase with osteoporosis deterioration, which indicates that oxidative stress plays a critical role in the progression of bone loss (Zhou et al. Many works suggest that oxidative stress plays a pivotal role in the development and progression of various types of osteoporosis (Munmun and Witt-Enderby 2021 Tao et al. ![]() Its pathogenesis involves dysregulated interactions between osteoblasts and osteoclasts, leading to bone formation and resorption imbalance (Cheng et al. It is estimated that the number of women over the age of 50 with osteoporosis is about twice that of men (Kim et al. Estrogen absence results in postmenopausal osteoporosis, inducing elevated bone metabolism and ultimately forfeiting bone trabecular connectivity and cortical osteoporosis (Ma et al. Osteoporosis is a systemic bone illness characterized by declined bone strength, bone mass, and elevation of deterioration of microstructure and brittleness, making patients to be more prone to fracture. Our research suggested that the PBC-modulated oxidative stress inhibition is responsible for activation of the process of osteogenic differentiation, providing a novel insight into the treatment of osteoporosis. ![]() Further study indicated that PBC exerted its beneficial function by suppressing oxidative stress-mediated alter bone metabolism to alleviate osteoporosis in vivo. Additionally, it was confirmed that PBC promoted osteogenic differentiation of MC3TE-E1 through inhibiting oxidative stress. Our results showed that PBC suppressed MC3TE-E1 resist oxidative stress to promote osteogenic differentiation. The ovariectomized rat model was set up successfully and HE staining were employed to examine femoral trabeculae tissue. Alizarin red staining and alkaline phosphatase staining were applied to examine osteogenic function and calcium deposits. Moreover, CCK-8 assay was conducted to detect cell viability. RT-qPCR and Immunofluorescence were used to measure the expression level of SOD2, SIRT1, intracellular oxidative stress levels and osteogenic markers proteins. The purpose of this study is to investigate the role of PBC in ovariectomized rat model and to explore the mechanism of osteogenic differentiation of MC3TE-E1 Cells. ![]() Probucol (PBC) is a potent cholesterol-lowering drug and has been studied extensively for its powerful antioxidative stress.
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